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KMID : 1037120160340020137
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The World Journal of Men′s Health
2016 Volume.34 No. 2 p.137 ~ p.144
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Development of an Improved Animal Model of Overactive Bladder: Transperineal Ligation versus Transperitoneal Ligation in Male Rats
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Kim Woo-Hyun
Bae Woong-Jin
Park Jung-Woo
Choi Jin-Bong
Kim Su-Jin
Cho Hyuk-Jin
Ha U-Syn
Hong Sung-Hoo
Lee Ji-Youl
Hwang Sung-Yeoun
Kim Sae-Woong
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Abstract
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Purpose: We compared a transperineal ligation model and a transperitoneal ligation model in male rats to determine which animal model of overactive bladder (OAB) was more useful based on cystometrography, estimations of oxidative stress, and measurements of pro-inflammatory cytokine levels.
Materials and Methods: Male rats were randomly divided into three groups (n=15 in each): the control group, the transperineal ligation group, and the transperitoneal ligation group. Four weeks after the ligation procedure, cystometrography was performed and oxidative stress, pro-inflammatory cytokine levels, and histologic changes were evaluated. Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine and superoxide dismutase, and pro-inflammatory cytokine activity was investigated by measuring levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α.
Results: The transperineal model led to results similar to those observed for the transperitoneal model, namely (1) increased voiding frequency and reductions in the non-voiding contraction interval and the maximal vesical pressure, (2) increased levels of oxidative stress markers, (3) increased pro-inflammatory cytokine levels, and (4) fibrotic changes in the bladder tissue.
Conclusions: We suggest that the transperineal procedure can be used as an alternative OAB model in male rats.
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KEYWORD
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Rats, Urinary bladder, overactive, Urinary bladder neck obstruction
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